Protective effects of sodium ferulate on the PC12 cells apoptosis induced by H_2O_2
- VernacularTitle:阿魏酸钠对H_2O_2诱导的PC12细胞凋亡的保护作用的探讨
- Author:
Xiying QU
;
Qifeng ZHU
- Publication Type:Journal Article
- Keywords:
sodium ferulate;
hydrogen peroxide;
PC12 cells;
apoptosis;
Par-4;
caspase-3
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the mechanism of the protective effects of sodium ferulate (SF) on apoptosis of PC12 cells induced by Hydrogen peroxide(H 2O 2). METHODS Based on the model of apoptosis of PC12 cells induced by H 2O 2, cell viability was tested by MTT assay. The expressions of mRNA and protein were tested by RT-PCR and Western blot. RESULTS Pretreatment with different concentrations of SF (25～250 ?mol?L -1) for 1h increased the survival rate of PC12 cells, decreased the expressions of par-4, caspase-3 mRNA and Par-4 protein, while there was no obvious change of the activity fregment of caspase-3 P20 and caspase-3 activity. CONCLUSION SF can prevent the PC12 cells against H 2O 2 neurotoxicity in a concentration-dependent fashion. The mechanism of protection is likely related to decreasing the Par-4 level, and the relation with Caspase-3 needs further study.
