Establishment of PAF receptor radioligand binding assay in mouse peritoneal macrophages
- VernacularTitle:小鼠腹腔巨噬细胞PAF受体放射配基受体结合分析方法
- Author:
Shanying PENG
;
Xueyu OUYANG
;
Wenjie WANG
- Publication Type:Journal Article
- Keywords:
PAF receptors;
radioligand binding assay;
mouse peritoneal macrophages
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish radioligand binding assay of PAF (platelet activating factor) receptors in mouse peritoneal macrophages and observe the characteristics of PAF receptors. METHODS PAF receptor radioligand binding was studied in intact adherent mouse peritoneal macrophages by -PAF. The radioactivity was counted with an LS6500 scintillation system. RESULTS The PAF receptor binding was shown to be saturable with an equilibrium K D of 3.2 nmol?L -1 and a B max of 100.2 fmol?1?10 6 cells -1. The competitive analysis showed that such specific binding could be inhibited by BN52021. CONCLUSION Utilizing the adherent character of macrophages, the binding ligands could be separated from non-binding ligands without negative pressure filtration, then cells could reserve fine activity, and PAF receptors could be near to physiological properties for screening of PAF antagonist.
