Effects of aging on testicular steroidogenic function in rats
- VernacularTitle:衰老对雄性大鼠睾丸类固醇合成功能的影响
- Author:
Yinghong SHAO
;
Yiming MU
;
Ming LI
;
Jiangyuan LI
- Publication Type:Journal Article
- Keywords:
aging;
testis;
testosterone;
steroidogenic acute regulatory protein;
17?-hydroxysteroid dehydrogenase
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the changes of testicular steroidogenic acute regulatory protein (StAR), 17?-hydroxys-teroid dehydrogenase (17?-HSD) and aromatase (P450arom) in elder SD rats, exploring the effects of aging on testicular steroidogenic function. Methods:The young male rats and old male rats were treated with human chorionic gonadotropin (hCG) for 3 times and then rat serum testosterone(T) and estradiol(E2) levels were determined by chemiluminescentenzyme immunoassay(CLIA) and the mRNA levels of StAR, 17?-HSD Ⅲ and P450arom were determined by RT-PCR. Results: (1) The levels of serum T and 17?-HSD Ⅲ mRNA of old SD rats were significantly lower than those of young rats before and after hCG stimulation (P
