Study on differentially expressed genes in Jurkat cells treated with phosphonoformate using DNA microarray
- VernacularTitle:应用基因表达谱芯片技术研究膦甲酸钠处理Jurkat细胞后的差异表达基因
- Author:
Yan LIU
;
Jun CHENG
;
Gang WANG
- Publication Type:Journal Article
- Keywords:
phosphonoformate;
immune regulation;
cDNA microarray
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the genes differentially expressed in gene expression in human lymphoma cell line Jurkat cells treated with phosphonoformate (PFA). Methods cDNA microarray technique was employed to detect the mRNA expressed in Jurkat cells treated with PFA or 0.9 percent sodium chloride, respectively. Results Among 1 152 genes there were 94 genes with different expression, of which 38 genes were upregulated and 56 genes were downregulated in Jurkat cells treated with PFA compared with those treated with 0.9 percent sodium chloride. Conclusions cDNA microarray technique was successfully used to screen the genes with different expression in Jurkat cells treated with PFA, and the results brought some new clues for the study of the immune regulation mechanism of PFA.
