Effect of fibronectin on infection of primary human fetal hepatocytes with HBV in vitro
- VernacularTitle:纤维结合素对HBV感染原代培养人胎肝细胞的影响
- Author:
Fang WANG
;
Yuming WANG
;
Xiaohong WANG
- Publication Type:Journal Article
- Keywords:
fibronectin;
hepatitis B virus;
human fetal hepatocytes;
cell culture
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective This experiment was carried out to study the effect of fibronectin on the infection of primary human fetal hepatocytes with hepatitis B virus (HBV) in vitro. Method Human fetal hepatocytes were inoculated with HBV positive serum in culture dish coated with fibronectin (Fn) in vitro. HBsAg and HBeAg in supernatant were assayed with ELISA, HBcAg in nuclei by immunohistochemistry, HBV DNA in cells by fluorescence quantitative PCR, and cccDNA in cultured cells by nest PCR. The results were compared with the cells cultured in dishes without fibronectin coating. Results HBsAg and HBeAg in supernatants were positive on the fifth day in dishes without Fn coating, and on the first day in dishes with Fn coating. HBcAg in nuclei was positive on the second and the first day in dishes without and with Fn coating, respectively, and the positive rates were about 10%～15% and 90%, respectively. HBV DNA was detected in cells on the fourth day and first day in dishes without and with Fn coating, respectively. CccDNA in cells was positive on the eighth day and the first day in dishes without and with Fn coating, respectively. Conclusion Fibronectin coating could facilitate HBV infection in primary fetal hepatocytes in vitro. The infection time of HBV was shortened, and the number of infected cells was increased.
