Effect of c-fos antisense oligonucleotide on the phenotypes of vascular smooth muscle cells in venous autograft
- VernacularTitle:反义c-fos核酸对自体移植静脉血管平滑肌细胞表型的影响
- Author:
Chunxi WANG
;
Zhiqiang HUANG
;
Zheng PENG
- Publication Type:Journal Article
- Keywords:
c-fos;
antisense oligonucleotide;
vascular smooth muscle cell;
restenosis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of c-fos antisense oligonucleotide on the phenotypes of vascular smooth muscle cells in venous autograftt. Methods The external jugular veins were grafted into abdominal aortic arteries in 20 Wistar rats which were divided into test group and control group randomly. The anastomosis and transplanted veins were coated with c-fos antisense oligonucleotide glue gel in the test group while the control group were merely coated with glue gel. The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later. Meanwhile the conversion status of the vascular smooth muscle cells were observed with electron microscope. Results The myo-endothelial structure was observed clearly in test group while it was obscure in control group; the expression of c-fos、c-myc、PCNA in vascular smooth muscle cells was significantly decreased in test group. Conclusions The c-fos antisense oligonucleotide can influence the phenotypes of the vascular smooth muscle cells in autografted veins and inhibit the cells’ proliferation. All these indicate that it is a prospective genetic prophylactic therapy for the restenosis of autografted veins.
