Effect of 17?-estradiol on the proliferation cycle of human mucoepidermoid carcinoma Mc3 cells
- VernacularTitle:17?-雌二醇对人涎腺黏液表皮样癌Mc3增殖周期的影响
- Author:
Xiaoxia LI
;
Junzheng WU
;
Qiaoqing LI
- Publication Type:Journal Article
- Keywords:
estradiol;
mucoepidermoid carcinoma;
CyclinD1;
P27 Kip1;
cell proliferation cycle
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 17?-Estradiol(E 2)on the proliferation cycle of human mucoepidermoid carcinoma Mc3 cells. Methods Mc3 cells were processed with various concentrations of E 2 for various durations. Then, cell proliferation and cell cycle distribution were examined by MTT assay and flow cytometry; CyclinD1 and P27 Kip1 expressions were assessed with immunohistochemistry. Results After being exposed to E 2 in concentrations of 10 -9,10 -8 or 10 -7mol/L for 5 days, the proliferation of the Mc3 cells was enhanced by 29%,54% and 59% respectively, CyclinD1 expression was increased by 16%,19% and 24%, And P27 Kip1 expression was decreased by 33%,30% and 55%, respectively. Compared with the control group, the number of cells in S phase increased by 11.3%,6.6% and 46.7% after the Mc3 cells were exposed to E 2 in the concentration of 10 -7mol/L for 12h,18h or 24h, and the proliferation index(PI) was also increased by 6.0%,3.6% and 205.0% respectively. Conclusion Physiological concentration of E 2 may promote Mc3 proliferation by increasing the expression of CyclinD1 and decreasing the expression of P27 Kip1, thereby stimulate the growth and development of the tumor.
