Receptor pathways involved in norepinephrine-induced intracellular ROS production in cardiac myocytes
- VernacularTitle:去甲肾上腺素刺激乳鼠心肌细胞内活性氧产生受体途径的研究
- Author:
Ji CHEN
;
Lan HUANG
;
Qian WU
- Publication Type:Journal Article
- Keywords:
norepinephrine;
myocardiocyte;
reactive oxygen species
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of norepinephrine (NE) on intracellular reactive oxygen species (ROS) production in cardiac myocytes and receptor pathways involved in ROS production. Methods DCFHDA fluorescent probe was used for the assessment of NE induced intracellular ROS production in cultured cardiac myocytes of the neonatal rats. Myocytes were exposed to NE, NE + prazosin, NE + propranolol, and NE + prazosin + propranolol at different time and doses. The changes of fluorescence intensity in cells were measured by flow cytometry. Results NE increased myocyte ROS production in time and dose dependent manners. NE induced ROS production was partially inhibited by prazosin or propranolol, and completely inhibited by prazosin and propranolol together in myocytes. Conclusion NE can induce intracellular ROS production in cultured cardiac myocytes of the neonatal rats in time and dose dependent manners through the mediation of both ? and ? adrenoceptors.
