Molecular epidemiology of TEM and SHV extended-spectrum ?-lactamases in Klebsiella pneumoniae and Escherichia coli clinical isolates from Chengdu,China
- VernacularTitle:产超广谱?-内酰胺酶大肠埃希菌及肺炎克雷伯菌的耐药基因序列分析
- Author:
Xuan CHEN
;
Xinjian FAN
;
Xiaoju L
- Publication Type:Journal Article
- Keywords:
lactamases;
Klebsiella pneumoniae;
Escherichia coli;
Drug-resistance, microbial
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate TEM and SHV ESBLs in Klebsiella pneumoniae and Escherichia coli clinical isolates from West China Hospital of Sichuan University and detect resistance of ESBLs-producing isolates. Methods The TEM and SHV ESBLs-encoding gene was amplified by PCR and was sequenced. And the MIC of eight antibiotics against the ESBLs-producing strains were detected by agar dilution. Results All strains were resistant to cefotaxime; eleven strains were resistant to aztreonam; two were resistant to ceftizidime; eleven, five and three were resistant to ciprofloxacin, amikacin and cefoxidine respectively; All strains were susceptible to imipenem. Ten strains of twelve ESBLs-producing strains carried bla SHV-2, two carried bla TEM-19. Conclusions ESBLs producers were mainly resistant to cefotaxime and aztreonam and most of them were multi-drug resistance; Cefotaxime resistance is partially due to production of SHV-2 and TEM-19 in this study.
