Effects of tetramethylpyrazine on proliferation of rat glomerular mesangial cells induced by lipopolysaccharide and levels of intercellular adhesion molecule-1
- VernacularTitle:川芎嗪对脂多糖诱导大鼠肾小球系膜细胞增殖及细胞间粘附因子-1表达的影响
- Author:
Xia WANG
;
Qixiong LI
- Publication Type:Journal Article
- Keywords:
tetramethylpyrazine;
mesangial cells;
proliferation;
intercellular adhesion molecule-1
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM : To investigate the effects of tetramethylpyrazine (TMP) on the proliferation of rat glomerular mesangial cells (GMCs) induced by LPS and the levels of intercellular adhesion molecule-1 (I CAM-1). METHODS : Different concentrations of TMP were put in the cultured rat GMCs in vitro after induced by LPS. Cell proliferation was assessed by MTT colorimetric assay and cell cycle was analyzed by flow cytometry. The levels of ICAM-1 were detected by immunohistology. RESULTS : The proliferation of MC induced by LPS was inhibited significantly by TMP with a dose-dependant manner. In the cell cycle, TMP increased the cells in G 0/G 1 phase and decreased the cells in S phase, and the ICAM-1 expression level was decreased. CONCLUSION : LPS-induced proliferation of MC is inhabited significantly by TMP. It blocks G 0/G 1 phase into S phase and inhibited the ICAM-1 expression.
