2D and 3D culture of human dental pulp stem cells
- VernacularTitle:人牙髓干细胞体外二维和三维培养的实验研究
- Author:
Yanhua HOU
- Publication Type:Journal Article
- Keywords:
dental pulp stem cells;
3D culture
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate human dental pulp stem cells and cultivate them in 2D and 3D.Methods Dental pulps were separated from the second premolars and digested by collagenase type Ⅰ and dispase.Dental pulp stem cells were obtained and identified.The proliferation of dental pulp stem cells cultivated in 2D and 3D was compared.Results There were clonogenic cells in human dental pulp.Some dental pulp stem cells could be differentiated into myocytes and odontoblasts.The cell density was increased to 4.12 times of the primary density in 2D culture and 11.06 times in 3D culture.After 3D culture,the biological properties of dental pulp stem cells remained.Conclusion Human dental pulp stem cells were successfully isolated and cultivated from the second premolars.3D culture is a suitable way to expand dental pulp stem cells.
