INDUCTION OF PROTECTIVE IMMUNITY AGAINST SCHISTOSOMA JAPONICUM WITH pcDNA3.1(+)/MLP NUCLEIC ACID VACCINATION
- VernacularTitle:日本血吸虫pcDNA3.1(+)/MLP核酸疫苗对家兔的保护性免疫作用
- Author:
Yan LIU
;
Jianhua XIAO
;
Li LIAO
;
Guqing ZENG
;
Yukuai ZHANG
;
Kegeng WANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Nucleic acid vaccination;
Protective immunity;
pcDNA3 1(+)/MLP(SJP)
- From:
Chinese Journal of Schistosomiasis Control
1989;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immune efficacy of nucleic acid vaccination in rabbits against Schistosoma japonicum with pcDNA3 1(+)/MLP(SJP) Methods The 24 New Zealand rabbits were randomly divided into 2 groups. The rabbits of experimental group were vaccinated by each quadriceps muscle of leg with pcDNA3 1(+)/MLP nucleic acid vaccination and the control group rabbits were vaccinated with pcDNA3 1(+). Each rabbit was immunized four times with 2 weeks interval. The rabbits were challenged 2 weeks after final DNA boosting by percutaneous infection with cercariae. Sixty days after infection the rabbits were sacrificed, the livers were investigated, and the worms and eggs in livers were counted. Blood sera were collected from rabbits and investigated. Results In the experimental group,the egg reduction rate was 28 10%. The rabbits of experimental group produced IgA, IgG 1, INF-?. Conclusion DNA vaccination with pcDNA3.1(+)/MLP could induce partial protective immunity against Schistosoma japonicum in rabbits.
