Detection of DNA tag sequences for rapid identification of Yersinia pestis
- VernacularTitle:鼠疫耶尔森菌DNA标识序列的鉴定及其应用研究
- Author:
Yanping HAN
;
Dongsheng ZHOU
;
Yajun SONG
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
DNA microarray;
DNA signature sequence
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the DNA tag sequences with the purpose of rapid and specific characterization of Y. pestis. Methods DNA microarray hybridization combined with PCR was used to perform genomic comparison between strains of Y. pestis and Y. pseudotuberculosis in order to screen and identify Y. pestis-specific genes. Results Twenty eight signature genes of Y. pestis were discovered. Three pairs of Y. pestis-specific primers were designed according to tag genes and proved to amplify the specific sequences of the target bacterium, showing no cross-reaction with the closely related Y. pseudotuberculosis and a large collection of genomic DNAs from other organisms. Conclusion DNA tag sequence is an ideal target for the rapid detection and identification of Y. pestis by PCR method.
