DNA microarray-based comparative genomic analysis of live plague vaccines
- VernacularTitle:鼠疫耶尔森菌活疫苗株的比较基因组学研究
- Author:
Dongsheng ZHOU
;
Yanping HAN
;
Erhei DAI
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
live plague vaccine;
DNA microarray;
comparative genomics
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and compare the genome differences among live plague vaccines prepared with different strains of the bacillus. Methods The whole-genome DNA microarray of Yersinia pestis was used as a tool to perform genomic comparison among live plague vaccines prepared with 19 different strains. Results Dozens of deletions and/or increased copies of the genomic fragments were identified in the studied vaccines of different strains. Conclusion The revealed genomic differences among the vaccines from different origins account for the variability of the immunogenic and protective potency of live plague vaccines. The whole-genome DNA microarray was also provesd to be an ideal tool for the pre-evaluation of a vaccine strain.
