Regulation of TGF-beta 1 and Collagen Expression by Antisense Oligodeoxynucleotide in Human Corpus Cavernosum Smooth Muscle Cells.
- Author:
Kwangsung PARK
1
;
Hye Joung KIM
;
Jeong Eun LEE
;
Seung Chul LEE
;
Soo Bang RYU
;
Hyun Chul LEE
Author Information
1. Department of Urology, Chonnam National University Medical School, Kwangju, Korea.
- Publication Type:Original Article
- Keywords:
Collagen;
Corpus cavernosum;
Transforming growth factor beta
- MeSH:
Blotting, Northern;
Collagen*;
Humans*;
Muscle, Smooth*;
Myocytes, Smooth Muscle*;
RNA, Messenger;
Transforming Growth Factor beta*;
Transforming Growth Factors
- From:Korean Journal of Andrology
1999;17(3):143-149
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Transforming growth factor (TGF)-beta 1 stimulates collagen synthesis in human corpus cavernosum smooth cells (HCCSMC). We studied whether an antisense TGF-beta 1 oligodeoxynucleotide changed TGF-beta 1 mRNA expression and its related collagen synthesis. MATERIALS AND METHODS: The HCCSMC were cultured primarily from three impotent patients. Expression of TGF-beta 1 mRNA was induced by phorbol 12-myristate 13-acetate (PMA) 0.2 micro gram/ml in the presence or absence of antisense TGF-beta 1 oligodeoxynucleotide (5'-GGGCGGCATGGGGGA-3') 20 micro gram/ml. The effect of the antisense oligodeoxynucleotide on TGF-beta 1 mRNA and collagen I synthesis were measured by RT-PCR or Northern blot analysis. RESULTS: The expression of TGF-beta 1 mRNA in response to PMA was inhibited by treatment with the antisense oligodeoxynucleotide. Collagen I expression was also induced by PMA treatment, and the antisense oligodeoxynucleotide downregulated this expression. CONCLUSIONS: Antisense TGF-beta 1 oligodeoxynucleotide can suppress the expression of TGF-beta 1 and TGF-beta 1-induced collagen I synthesis in HCCSMC.
