Promotion of morphine on rat purine nucleotides catabolism and its possible mechanisms
- VernacularTitle:吗啡对大鼠嘌呤核苷酸分解代谢的促进作用及其可能的作用机制
- Author:
Mujie KAN
;
Min HONG
;
Hong ZHOU
;
Lin GAO
- Publication Type:Journal Article
- Keywords:
morphine;
xanthine oxidase;
uric acid;
gene expressio n;
small intestine
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of morp hine on purine nucleotides catabolism and its possible mechanisms. METHO DSRats were administered with morphine by intraperitoneal injection with increasing dose to develop ad diction model. The determination of uric acid concentration in plasma and the ac tivities of xanthine oxidase (XO) in the plasma and the small intestine were per formed. RT-PCR was used to examine the expressing level of key enzyme of purine nucleotides catabolism,XO mRNA. ?-actin was used as control gene in RT-PCR s tudy. RESULTSThe concentration of uric acid in plasma was sign ificantly increased in morphine-pretreated rats compared with control(P
