The Biological Characteristics of Different Generation of Osteoblasts
- VernacularTitle:不同代数大鼠成骨细胞生物学特性的实验研究
- Author:
Yudong CHEN
- Publication Type:Journal Article
- Keywords:
different subculture;
osteoblasts;
ALP;
mineralization nodules
- From:
Journal of Zhejiang Chinese Medical University
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the discrepancy on biological characteristics of different subculture osteoblasts in vitro.[Methods] Compare the morphology,AKP activities and mineralization nodules of 1,3,5,7 and 9 subculture osteoblasts,and make statistic of the result.[Results] There was no discrepancy within 1,3 and 5 subculture osteoblasts,but the 7,9 subculture was significantly worse than subculture 1 in the morphology,AKP activities and mineralization nodules.[Conclusion] The osteoblasts within 5 subculture were suitable for experiment in vitro.
