Effects of rhCNTF on axoplasmic transport following incomplete injury of optic nerves of adult rats
- VernacularTitle:睫状神经营养因子对大鼠视神经不全损伤后轴浆运输的影响
- Author:
Can LI
;
Yi WANG
- Publication Type:Journal Article
- Keywords:
optic nerve;
incomplete injury;
ciliary neurotrophic factor;
horseradish peroxidase;
axoplasmic transport
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of ciliary neurotrophic factor (CNTF) on the recovery of axoplasmic transport following incomplete injury of the optic nerves (ON) of the adult rats. Methods Moderate injury of the optic nerves of 50 adult female rats was induced by crushing the optic nerves for 10 seconds using hemostatic forceps. Recombinant human CNTF (rhCNTF) was injected into the vitreous cavity of CNTF treated group at 0, 1, 2, 4, and 8 weeks after injury. However, the same volume of distilled water was injected into the vitreous cavity of the control rats. The changes of axoplasmic transport were assessed by anterograde labelling combined with horseradish peroxidase (HRP) at 1, 2, 4, 8, and 12 weeks after injury. Results The labelling density at ON distal to the site of injury by HRP obviously decreased at 1 and 2 weeks after injury, and gradually enhanced at 4 weeks and peaked at 8 weeks after injury. The recovery ratios of axoplasmic transport in CNTF treated group and control group at 8 weeks after injury were 31 86% and 12 87%, respectively. No HRP reaction product was observed in the superior colliculus at 1 and 2 weeks after injury. There was significant difference of the HRP anterograde labeling density in ON and the superior colliculus between the CNTF treated group and the control group during 4 to 12 weeks after injury ( P
