Development of whole-genome DNA microarray of Yersinia pestis and its validity in comparative genomic analysis
- VernacularTitle:鼠疫耶尔森菌全基因组DNA芯片的研制及用于比较基因组学分析
- Author:
Dongsheng ZHOU
;
Yanping HAN
;
Erhei DAI
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
whole-genome DNA microarray;
comparative genomics
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a whole-genome DNA microarray based on the genomic sequences of Y. pestis CO92 and 91001 and its use in comparative genomic analysis. Methods A total number of 4 005 genes of Y. pestis were amplified by PCR and printed onto glass slides in duplicate. Fluorescently labeled probes were prepared by marking genomic DNAs with random hexamers and Klenow. Labeled DNAs were hybridized with the microarrays by the method of two-fluorescence comparative hybridization. Three sets of two-fluorescence hybridizations were performed to examine the absence/presence of each gene. Results The results agreed with those derived from the in silico genomic comparison. Conclusion The results demonstrate that the microarry can be a useful tool for comparative genomic analysis of Y. pestis.
