Expression of histamine H_1 receptor gene in hepatocarcinoma of rats induced by dimethylamino-azobenzene
- VernacularTitle:二甲基氨基偶氮苯诱导大鼠肝癌中组胺H_1受体的基因表达
- Author:
Li LI
;
Naiping WANG
;
Zong NING
;
Jinbin WEI
;
Linquan ZANG
- Publication Type:Journal Article
- Keywords:
rats;
hepatocarcinoma;
histamine H_1 receptor;
gene expression;
reverse transcription-polymerase chain reaction
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the mRNA expression of histamine H_1 receptor in hepatocarcinoma of rats. METHODS Dimethylamino-azobenzene (DAB) was used to induce hepatocarcinoma in rats. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was adopted to analyse the relative expression of histamine H_1 receptor. And the base sequence of its PCR product was detected. RESULTS The relative mRNA expression of histamine H_1 receptor was significantly decreased in hepatic carcinoma tissue, compared with that part far from cancer and control group (P
