The effects of epimedium pubescens flavonoids combined with diethylstilbestrol on ovariectomy induced osteopenia in rats
- VernacularTitle:淫羊藿总黄酮与雌激素合用对去卵巢大鼠骨质疏松的影响
- Author:
Bilian XU
;
Tie WU
;
Liao CUI
;
Yuyu LIU
- Publication Type:Journal Article
- Keywords:
epimedium pubescens flavonoids;
diethylstilbestrol;
ovariectomized rat;
osteoporosis;
bone histomorphometry
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To determine whether epimedium pubescens flavonoids(EF) in combination with diethylstilbestrol(DES) can completely prevent bone loss in ovariectomized rats. METHODS Sixty Sprague-Dawley female rats at age of 4 months were divided into six groups. Ten rats were sacrificed at age of 4.5 months before operation. The other rats were sham-operated and treated orally with vehicle or ovariectomized (OVX) and treated orally with either vehicle, or diethylstilbestrol(DES) at 22.5 ?g?kg -1 ?d -1 , or EF at 300 mg?kg -1 ?d -1 , or EF 300 mg?kg -1 ?d -1 in combination with DES 22.5 ?g?kg -1 ?d -1 for 90 days. Double in vivo fluorochrome labeling was administrated. The undecalcified longitudinal proximal tibial metaphyseal sections were used for [FQ(9*2。46,X-WZ]-the bone histomorphometric analysis. RESULTS DES prevented OVX-induced bone loss and decreased body weight gain and total serum cholesterol,but it increased uterine luminal epithelial thickness.Combination of EF with DES completely prevented OVX-induced bone loss and did not increase uterine luminal epithelial thickness.The effect of the combination in preventing bone loss was more significant than that of DES. CONCLUSION EF in combination with DES showed synergistic effect on prevention of osteoporosis induced by ovariectomy.-
