Effects of sodium ferulate on hepatocyte growth inhibited by transforming growth factor ?_1
- VernacularTitle:阿魏酸钠对转化生长因子?_1抑制肝细胞增殖作用的影响
- Author:
Jin HUANG
;
Jinhong HU
;
Zhen CAI
;
Hongjie SONG
;
Wanguo ZHANG
- Publication Type:Journal Article
- Keywords:
sodium ferulate;
transforming growth factor ?_1;
hepatocyte;
reactive oxygen species
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To research effects of sodium ferulate(SF) on transforming growth factor ?_1 inhibited hepatocyte growth. METHODS Human cultured hepatocytes(L02 cells) served as control group ,TGF?_1(5 ?g?L -1 ) was used to inhibited L02 cells to construct the cell model. MTT methods were used to study the growth inhibitory effect of TGF?_1 on L02 cells. DNA synthesis was analyzed by measuring 3H-TdR incorporation. The effects of SF on the changes of cell cycle were analyzed by flow cytometry. Generation of intracellular ROS was determined by the emission of fluorescence in L02 cells proloaded with DCFH-DA fluoresin and then treated them with TGF?_1 or SF. After 4h,control, TGF?_1 and TGF?_1+SF-treated cells were analyzed by flow cytometry. RESULTS ①Incubated in the presence of TGF?_1 for 24 h, 67.93% of L02 cells survived (P
