Quantitive analysis of the inhibition of HCV IRES mediated HCV core protein expression in cells by inhibitor RNA
- VernacularTitle:抑制性RNA对细胞内HCV IRES介导HCV核心蛋白表达抑制作用的定量分析
- Author:
Xuesong LIANG
;
Yongxing ZHOU
;
Jianqi LIAN
- Publication Type:Journal Article
- Keywords:
Ribosomes;
Hepatitis C-like viruses;
Microscopy, Confocal;
RNA, visal
- From:
Chinese Journal of Infectious Diseases
1999;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibition of HCV IRES mediated HCV core protein expression in cells by inhibitor RNA. Methods Plasmid pcRz-IRNA, a eukaryotic expression vector with IRNA and two self cleavage ribozyme overhang at both sides respectively, was constructed and co-transfected with pcHCVcluc (containing HCV NCR, core and Luc genome) into the HHCC cell line (Human Hepatocellular Carcinoma cell line). Immunoflurescence tests were applied to detect the co-transfected cells, which were thereafter analysed with confocal microscope quantitatively. Luciferase activity was valued using Luc Assay System (Promega). Results The cotransfected cells expressed HCV core protein, and the fluorecein in which was reduced significantly in comparison with control. Conclusions IRNA can inhibit the expression of HCV IRES mediated core protein in the cotransfected cells.
