Activation of NF-?B and anti-inflammation mechanism of pyrrolidine dithiocarb amate in rats with acute colitis
- VernacularTitle:大鼠急性肠炎早期核因子-?B活化以及吡咯烷二硫氨基甲酸的抗炎作用
- Author:
Ken CHEN
;
Youming LONG
;
Lei LAN
;
Jieshun ZHENG
;
Hui WANG
- Publication Type:Journal Article
- Keywords:
rats;
model;
nuclear factor;
colitis;
tr initrobenzene sulfonic acid
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate DNA-binding activity of nuclear factor ka ppa B (NF-?B) and pyrrolidine dithiocarbamate (PDTC) anti-inflammation effect and mechanism in trinitrobenzene sulfonic acid (TNBS)-induced acute colitis in rats. METHODS: Acute colitis was induced by instilling TNBS/ ethanol into the lumen of the rat colon. Rats were randomized into PDTC-treat g roup (including group P10,P25,P50,P100), TNBS/ethanol group and normal control g roup. The rats in PDTC-treated group were injected intraperitoneally with PDTC at the dosages of 10, 25, 50, and 100 mg?kg -1 , respectively. Rats wer e killed at 4 h after enema. Colonic inflammation, the ratio of wet/dry weig ht and MPO, SOD, and MDA activity were detected. DNA-binding activity of NF-? B was assessed by EMSA. RESULTS: NF-?B activity in colon homog enate was increased markedly at acute colitis in rats. PDTC attenuates the devel opment of rat colonic inflammation but not by reducing the NF-?B activity. CONCLUSION: NF-?B is activated in rats with acute colitis, which may be a mechanism of self-protection. PDTC can attenuate the development of in flammation.
