The application of temporal temperature gradient gel electrophoresis in the detection of mitochondrial gene mutations
- VernacularTitle:时相温度梯度凝胶电泳在线粒体基因突变检测中的应用
- Author:
Duanjun TAN
;
Lingling LIU
;
Yi WEN
- Publication Type:Journal Article
- Keywords:
neoplasms;
temporal temperature gradient gel electrophoresis;
DNA, mitochondrial;
mutation
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the potential utility of temporal temperature gradient gel electrophoresis (TTGE) in detecting homoplasmic and heteroplasmic mtDNA mutations in patients with cancers. Methods The entire mtDNA of tumor and corresponding normal tissues from total 117 patients were amplified by PCR, and the somatic mutations were screened by TTGE. DNA fragments showing different band patterns in normal and tumor tissues in TTGE were sequened to identify the exact mutations. Results 160 fragments harbouring mtDNA mutations were found by TTGE in total 3 654 fragments. Compared with the direct squencing results, the sensitivity and specificity of TTGE in mutation detection were 96.27% and 94.05% respectively. Conclusion TTGE is an effective method for screening acquired homoplasmic mitochondrial gene mutation and its different percentage.
