In vitro study of incorporation of p-boronophenylalanine (BPA) by glioma cells and its relationship with cell cycle
- VernacularTitle:星形胶质瘤细胞摄取BPA的时间及细胞周期相关性研究
- Author:
Ruifeng CAO
;
Xiang ZHANG
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
glioma;
boron neutron capture therapy;
cell cycle
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the incorporation of BPA, which was synthesized by ourselves, by two different glioma cell lines, and to observe its relationship with the time of cultivation and cell cycle. Methods Two glioma cell lines of C6 and SHG-44 were studied and the primarily cultured rat astrocytes were used as control. The growth curves of the two glioma cell lines and rat astrocytes were plotted, and their doubling time was identified respectively from the curves. All three kinds of cells were incubated in a culture medium, in which 10 B concentration was 50?g/ml for 4h, 8h, 16h, 20h or 24h. Boron concentration in the cells was measured by induced couple plasma atomic emission spectroscopy (ICP-AES) after respective culture period. After 24h of incubation, the cells in the G 0 /G 1 phase and those in the G 2 /M phase were isolated by flow cytometry, and boron concentration in each fraction was obtained by ICP-AES. Results The doubling time was 18.5h for both C6 and SHG-44 cells, but 28h for the astrocytes. The boron concentration in glioma cells was constantly higher than that in astrocytes throughout the experiment(P
