RAPID BIOCHEMICAL SYSTEM FOR IDENTIFICATION OF AN AEROBIC BACTERIA
- VernacularTitle:厌氧菌快速生化鉴定系列
- Author:
Qinglin LI
- Publication Type:Journal Article
- From:
Chinese Medical Equipment Journal
1989;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
We reported here a rapid biochemical system for identificationm of anaerobic bacteria by directly detecting cnzyme produced by cultured anaerobic colonial cells. Anaerobic bacteria suspension was prepared in pH7.4 bouillon yeast solution, a series of different substrates was added,then the mixture was incubated in 37~C for 4-6 hours.Various specific reactions could be shown by adding indicator or reagents.Our system is modified fromAPI 20 and similar to AN-IDENT,RAPID- ANA rapid enzyme methods 12 anaerolic strains of international standard were detected usind this system,the corresponding rate was 92.3%,while the average corresponding rate arrived at 87.5% after detecting 192 strains isolated in hospitals. We coneluded from the results thot this system is a rapid,simple and specific method for identification of anaerobic bacteria and can be applied to clinical and experimental use.
