To screen binding protein of hepatitis B virus core promoter with phage display technic
- VernacularTitle:应用噬菌体展示技术筛选乙型肝炎病毒核心启动子结合蛋白
- Author:
Zhongdong ZHANG
;
Jun CHENG
;
Yanwei ZHONG
- Publication Type:Journal Article
- Keywords:
phage display;
HBV core promoter;
screening
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the HBV core promoter binding protein, and to investigate their potential role in the replication of HBV DNA. Methods By using HBV core promoter being used as a selective molecule, the T7select human liver cDNA library was biopanned and the positive clones were selected. Results After phage display screening, the positive plaques was amplified and then cloned into the pGEM-Teasy vector. Six positive plaques were chosen for DNA sequencing. The binding protein of HBV core promoter was identified as caboxypeptidase N(CPN) by BLAST. Conclusion The results suggest that phage display screening of binding protein of HBV core protein provides a new approach to study the replication mechanism of HBV DNA.
