Screening of binding protein of hepatitis C virus NS4A by phage display from human liver cDNA library
- VernacularTitle:应用噬菌体展示技术筛选丙型肝炎病毒非结构蛋白NS4A结合蛋白
- Author:
Yanwei ZHONG
;
Jun CHENG
;
Zhongdong ZHANG
- Publication Type:Journal Article
- Keywords:
phage display;
hepatitis C-like viruses;
viral nonstructural proteins;
gene library
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the HCV NS4A binding protein. Methods By using HCV NS4A as a solidified selective molecule, the T7 select human liver cDNA library was biopanned and the positive clones were selected. After screening, the positive plaques was amplified and then cloned into the pGEM-Teasy vector. Two positive plaques were chosen for DNA sequencing. Results The binding protein of HCV NS4A was identified as mitogen-activated protein kinase (MAPK)-activated protein kinase 5 (MAPKAPK5) by BLAST. Conclusion This approach provides a new way for the study of the pathogenic mechanism of HCV infection.
