Screening of binding protein of surface protein promoter Ⅱ DNA of hepatitis B virus by phage display from human liver cDNA library
- VernacularTitle:应用噬菌体展示技术筛选乙型肝炎病毒表面抗原基因启动子Ⅱ结合蛋白
- Author:
Yanjie YANG
;
Jun CHENG
;
Dongfeng CHEN
- Publication Type:Journal Article
- Keywords:
phage display;
hepatitis B virus;
antigens, surface;
promoter regions (genetics);
screening
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the HBV SPⅡ promoter DNA-binding protein, and to investigate its potential role in the regulation of replication and expression of HBV DNA. Methods By using HBV SPⅡ biotinylated promoter DNA as a selective molecule, the T7 select human liver cDNA library was biopanned and the positive clones were selected. After screening, amplification of positive plaques was performed for inserted DNA fragment and then they were cloned into the pGEM-Teasy vector. Results Four positive plaques were chosen for DNA sequencing. The binding protein of HBV SPⅡ promoter was demonstrated as nicotinamide adenine dinucleotide (NADH) dehydrogenase 4 by BLAST. Conclusion The result suggests that this approach may provide a new tool for the study of replication and expression mechanism of HBV DNA.
