Effects of NF-kappa B inhibition by pyrrolidine dithiocarbamate on tissue pro-and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis
- VernacularTitle:NF-?B抑制剂对烫伤脓毒症大鼠致炎/抗炎细胞因子表达的影响
- Author:
Yongming YAO
;
Sheng YAO
;
Jinsong CHEN
- Publication Type:Journal Article
- Keywords:
burns;
tumor necrosis factor;
interleukin-10;
NF-kappa B;
Staphylococcus aureus;
sepsis
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of inhibition of nuclear factor-kappa B (NF-?B) by pyrrolidine dithiocarbamate (PDTC) on tissue pro- and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis and its potential regulating mechanism. Methods Thirty-four male Wistar rats were randomly divided into normal control group (n=6), scald control group (n=6), postburn sepsis group (n=12),and PDTC treatment group (n=10). Tissue samples from the liver, kidneys and lungs were collected to determine tumor necrosis factor-? (TNF-?) and interleukin-10 (IL-10) mRNA expression as well as their protein levels. Results Both TNF-? mRNA and protein expressions in the liver, kidneys and lungs of postburn septic animals were up-regulated rapidly at 0.5-2 hours (P
