Effects of angiostatin gene transfection on biological behaviors of bladder carcinoma BIU-87 cell line
- VernacularTitle:Angiostatin基因转染对膀胱癌BIU-87细胞系生物学行为的影响
- Author:
Kai ZHENG
;
Shaozeng ZHANG
;
Yangmin WANG
- Publication Type:Journal Article
- Keywords:
angiostatin;
transfection;
bladder neoplasms;
neovascularization, pathologic
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and effects of extragenous angiostatin gene in human bladder carcinoma BIU-87 cell line. Methods A full human angiostatin gene was cloned into expression vector pcDNA3.1(+) and transfected into human bladder carcinoma BIU-87 cells by lipofectamine, and the positive clone was screened by G418. The growth characteristics of BIU-87 cells before and after transfection were compared.The expressions of angiostatin protein in BIU-87 cells were examined by immunofluorescence assay, immunohistochemistry and Western blotting, and the activities of angiostatin protein were examined by endothelial cell inhibition assay and the chicken-embryo chorioallantoic membrane assay. Results The expression of extragenous angiostatin gene had no inhibitory effect on the growth of BIU-87. The transfected cells could inhibit the umbilical vein endothelial cell growth in vitro and chicken-embryo choriallantoic membrane angiogenesis in vivo. Conclusion The results indicated that the angiostatin gene clones could be transfected into BIU-87 bladder cancer cells, and angiostatin could inhibit endothelial cell proliferation and neovascularization.
