ATP sensitive potassium channels are involved in the protective effect of ischemic preconditioning on spinal cord in rabbits
- VernacularTitle:ATP敏感性钾通道参与兔脊髓缺血预处理的保护作用
- Author:
Hanfei SANG
;
Qibing MEI
;
Gu GONG
;
Lixian XU
;
Lize XIONG
;
Xingbin YANG
- Publication Type:Journal Article
- Keywords:
ATP sensitive potassium channels;
ischemic preconditio ning;
spinal cord;
ischmic and reperfusion injury;
rabbits
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate whether ATP sensitive pota ss ium channels are involved in the protective effects of ischemic preconditioning on spinal cord in rabbits. METHODS Twenty seven male New Zealands white rabbits were randomly assigned to 3 groups (9 in each group):ischemia gro up(IC)、ischemic preconditioning group (IPC) and glibenclamide + ischemic precon ditioning group(G+I). In IC group, spinal cord ischemia was induced by an infrar enal aorta clamping for 20 min; IPC group underwent a 6 min ischemic preconditio ning followed by 30 min of reperfusion before the 20 min clamping; G+I group wer e administered glibenclamide (an ATP sensitive potassium channel blocker, 2 mg? kg -1 )intravenously 20 min before the ischemic preconditioning. Neurologic function was scored at 8,12,24 and 48 h after reperfusion. All animals were sa crificed at 48 h after reperfusion and the spinal cords (L 5～7 ) were remov ed for histopathologic study. RESULTS The neurologic function sco res in IPC group at each observe interval were higher than those in IC group and G+I group (P
