The pharmacological preconditioning of nitroglycerin and buprenorphine used in combination against myocardial ischemia in rats
- VernacularTitle:硝酸甘油与丁丙诺啡合用抗大鼠心肌缺血的药理性预适应研究
- Author:
Yanjun KONG
;
Yanxia LIU
;
Jianshi LOU
- Publication Type:Journal Article
- Keywords:
nitroglycerin;
buprenorphine;
pharmacological precondit ioning;
delayed cardioprotection
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the delayed cardioprotection eff ec ts of pharmacological preconditioning of nitroglycerin (NG) and buprenorphine (B U) used alone or in combination on myocardial ischemia in rats. METHODS Male Wistar rats were randomized to 5 groups. Normal saline (NS) was inf used intravenously with the same volume of NG and BU in Sham group. The anterior mesenteric artery was ligated for 10 min in delayed ischemic preconditioning (D IP) group. BU (1 0 mg?kg -1 ) and NG (0 3 mg?kg -1 ) was administere d intravenously alone or in combination in BU, NG and B+N group respectively. Th e ischemia and reperfusion injury were induced by ligation of left arterior desc ending coronary artery for 30 minutes and followed 2 hours reperfusion at 24 h a fter first operation. Heart rate, blood pressure, ECG ST-segment and arrhythmia s were recorded continuously throughout the whole test. The plasma LDH and CK we re measured. on 30min after ischemia and 2 h after reperfusion. HE and TTC stain ing were performed to determine the myocytes necrosis at the end of test. RESULTS Compared with sham group,DIP group reduced ST-segment during ischemia ( P
