Effect of ketamine on ATP-sensitive K~+ currents in airway smooth muscle cells of asthmatic rat
- VernacularTitle:氯胺酮对哮喘大鼠气管平滑肌细胞ATP敏感钾电流的影响
- Author:
Hongguang NIE
;
Yong CUI
;
Dongyun HAN
;
Lei CHEN
;
Jinming LI
- Publication Type:Journal Article
- Keywords:
ketamine;
ATP-sensitive potassium currents;
airway smo oth muscle;
asthma
- From:
Chinese Pharmacological Bulletin
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effect of ke tamine on ATP-sensitive K + currents (I K ATP ) in single airway smooth muscle (ASM) cells of asthmatic rat. METHODS Single ASM cells of asthmatic rat were isolated with enzymatic dissociation technique. Effect of ketamine on I K ATP in single ASM cells was studied using the whole-cell configu ration of patch clamp technique. RESULTS Ketamine opened the ATP -sensitive K + channel (K ATP channel) in a dose-dependent manner. When the concentrations of ketamine were 1?10 -7 ,1?10 -6 ,1?10 -5 and 1?10 -4 mol?L -1 , the amplitude values of I K ATP were increased to 63 86?19 33 pA/pF(n=8,P
