Homologous analysis of E.coli O_(157)∶H_7 by pulsed-field gel electrophoresis and random amplified polimorphic DNA
- VernacularTitle:肠出血性大肠埃希菌(O_(157)∶H_7)的基因同源性的分析
- Author:
Jinchuan YANG
;
Huaiqi JING
;
Hongwei LI
- Publication Type:Journal Article
- Keywords:
Electrophoresis, gel, pulsed field;
Random amplified polymorphic DNA technique;
Polymerase chain reaction;
Bacterial toxins;
Escherichia E. coli O 157∶H 7;
Gastrointestinal hemorrhage
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the molecular epidemiological characteristics of E. coli O 157∶H 7 of Xuzhou, Jiangsu. Methods The virulence gene spectrum of E. coli O 157∶H 7 strains were analyzed by PCR and the homology of E. coli O 157∶H 7 strains were detected by PFGE and RAPD. Results In all E. coli O 157∶H 7 strains isolated from epidemic area, 100% possess Hly and eaeA gene, 95.35% possess SLT 2 gene, and 11.63% possess SLT 1 gene. The PFGE spectrum showed that the strains isolated from epidemic area were distinctively different from the strains isolated from Japan, and similar to but not identical with the standard strain 882364. The PFGE spectrum of strains isolated from epidemic area patients were identical with those of strains isolated from excrements of poultries, domestic animals and insect intestine.Conclusions Poultries and domestic animals which carry E.coli O 157∶H 7 could be the source of infection. PFGE could be used to analyze E.coli O 157∶H 7 and played an important role in epidemiology study. The results showed that the method of analysis of E. coli O 157∶H 7 by RAPD was convenient and time saving.
