Effect of connective tissue growth factor on transdifferentiation of human renal tubular epithelial cell
- VernacularTitle:结缔组织生长因子对人肾小管上皮细胞转分化影响的研究
- Author:
Chun ZHANG
;
Zhonghua ZHU
;
Anguo DENG
- Publication Type:Journal Article
- Keywords:
Connective tissue growth factor;
Renal tubule;
Epithelial cell;
Transdifferentiation
- From:
Chinese Journal of Nephrology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cells (HKC).Methods Cultured HKC were divided into 3 groups: (1) negative control; (2) low dose CTGF treated (rhCTGF 2. 5 ng/ml); (3) high dose CTGF treated (rhCTGF,5. 0 ng/ml). Expression of ?-smooth muscle actin (?-SMA) and fibronectin(FN) mRNA were measured by RT-PCR. Indirect immunofluorescence and flow cytometry methods were used to assess the level of intracellular ?-SMA protein. Concentration of FN secreted into the media was determined by ELBA. Results Upon the stimulations of different concentrations of rhCTGF,expression of ?-SMA and FN mRNA increased markedly(P
