Effects of 3 traditional Chinese formulas of herbal medicine in prevention and treatment of diabetic rats induced by streptozotocin
- VernacularTitle:芪苓汤、八味地黄丸、柴苓汤抗糖尿病发病和降血糖的探讨
- Author:
Zhiming WEN
- Publication Type:Journal Article
- Keywords:
pharmacodynamics;
diabetic rats;
streptozotocini;
herbal medicine;
cell-mediated immunity;
immunohistochemistry
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the mechanisms of Chinese herbal medicin e formulas (Qi-ling-tang, Ba-wei-di-huang-wan and Chai-ling-tang) in the p revention and treatment of diabetes. METHODS: Wistar rats were d ivided into five groups and treated with Qi-ling-tang (the group QLT), Ba-wei -di-huang-wan (the group BWDHW), Chai-ling-tang (the group CLT), water (the group model) and control, respectively. The rats in groups QLT through model we re injected with 30 mg?kg -1 /daily streptozotocin (STZ) for five days beginning on day 8. The glucose concentration, fructosamine, insulin, total-pr otein, albumin and the percentage of W3/25 and OX8 T-cells in serum as well as the body weight of the rats were measured. Tissue sections of paraffin-embedde d pancreas of the rats were observed after staining with HE, AZAN and immunohist ochemistry. RESULTS: After STZ injection, the diabetic incidence of the rats in groups QLT, BWDHW and CLT were significantly lower than that in the group model, especially in the group BWDHW was only 21% by study day 10. A significant decrease in blood glucose concentration was found in groups BWDHW (25%) and CLT (18%) from study day 15 to 26. The percentage of W3/25 cell s in groups QLT and BWDHW was higher than that in the control group. The averag e area of islets and the area of bate cells were greater in groups QLT, BWDHW an d CLT than in the group model. CONCLUSION: Three formulas retard the development of hyperglycemia of rats induced with multiple low doses STZ by increasing the cell mediated immunofunction. The formulas Ba-wei-di-huang-w an and Chai-ling-tang can reduce hyperglycemia.
