JAK2/STAT3 signaling pathway mediating proliferation and apoptosis of vascular smooth muscle cells in rats
- VernacularTitle:JAK2/STAT3信号通路介导大鼠血管平滑肌细胞的增殖与凋亡
- Author:
Mei TANG
- Publication Type:Journal Article
- Keywords:
vascular smooth muscle cell;
signal transducers and activators of transcription factor 3;
protein tyrosine kinase;
proliferation;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between STAT3 signal transduction pathway and proliferation as well as apotosis of vascular smooth muscle cells in rat.Methods VSMCs of rats were treated with AG490(a selective JAK2 inhibitor).MTT assay and flow cytometry were used to measure the proliferation and apoptosis.(RT-PCR) was performed to detect the levels of JAK2 mRNA expression from VSMCs.The expression of STAT3,(P-STAT3),JAK2,P-JAK2,cyclin D1 and BCL-2 proteins was measured by Western blot.Results High expression of JAK2 mRNA was detected in VSMCs and there was obvious decline of JAK2 mRNA after AG490 treating((P
