Evaluation of Nested Multiplex PCR in the Diagnosis of Malaria Infection.
- Author:
Young Ree KIM
1
;
Hyun Kyung CHOI
;
Jun Soo BAE
;
Jang Su KIM
;
Jung Ah KWON
;
Inbum SUH
;
Chae Seung LIM
;
Yoo Seok KHO
;
Kyung Ok LEE
;
Yoo Sung HWANG
Author Information
1. Department of Laboratory Medicine, College of Medicine, Korea University, Korea. malarim@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Nested multiplex PCR;
Malaria;
Plasmodium vivax;
Plasmodium falciparum
- MeSH:
Diagnosis*;
Discrimination (Psychology);
DNA;
Edetic Acid;
Electrophoresis;
Humans;
Korea;
Limit of Detection;
Malaria*;
Multiplex Polymerase Chain Reaction*;
Plasmodium falciparum;
Plasmodium vivax;
Polymerase Chain Reaction;
Sensitivity and Specificity
- From:The Korean Journal of Laboratory Medicine
2002;22(6):447-451
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: A peripheral blood smear has been the gold standard method for the diagnosis of malaria infection. Recently, many other methods have been introduced, although having inferior sensitivity and specificity to peripheral blood smears. We evaluated Neodin malaria PCR kit and its applicability in clinical settings. METHODS: Samples from seventy patients who visited Korea University hospital were used for evaluation. DNA from EDTA blood was tested in nested multiplex PCR and 470 bp for Plasmodium vivax or 340 bp for Plasmodium falciparum was confirmed after electrophoresis. The detection limit was determined by dilution of malaria positive blood with normal blood. RESULTS: Thirty-five cases of P. vivax and 10 cases of P. falciparum were noted. Except for a case of falciparum malaria, all positive cases were consistent with the peripheral blood smear results. Detection limit was 3.6 parasite/microL. CONCLUSIONS: Neodin malaria nested multiplex PCR has high sensitivity and the ability for species discrimination and may be available in the diagnosis of malaria infection.
