CONSTRUCTION OF AN INDUCIBLE VECTOR CARRYING GREEN FLUORESCENT PROTEIN
- VernacularTitle:携带绿色荧光蛋白的可诱导性真核表达载体的构建及其表达
- Author:
Jianyong ZHENG
;
Kaizong LI
;
Weizhong WANG
- Publication Type:Journal Article
- Keywords:
gene therapy;
green fluorescence protein;
carcinoma, hepatocellular
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the inducible vector carrying green fluorescence protein(GFP). Methods We constructed an inducible vector pMD-GFP, including GFP cDNA, which allowed controlled expression of protein upon addition of 100 ?mol/L Zinc as an external inducer. The whole length of GFP cDNA were transfected into human hepatocellular cancer cells HCC-9204 by the method of lipofectin transfection. The expression of GFP was observed under fluorescence microscopy. Results The inducible vector carrying green fluorescence protein was successfully constructed. The observation under fluorescence microscopy showed that green fluorescence was spread in entire HCC-9204 cells transfected with GFP gene. Conclusion This new kind of the inducible vector could serve as a new tool and method for observing the growth and metastasis of neoplasm.
