ROLE OF GRANULOCYTE COLONY-STIMULATING FACTOR IN REGULATING DENDRITIC CELL SUBSETS AND ITS EFFECT ON IMMUNE RESPONSE
- VernacularTitle:G-CSF对树突状细胞的调节作用及其对免疫应答的影响
- Author:
Jingshi ZHOU
;
Kefeng DOU
;
Rong FU
- Publication Type:Journal Article
- Keywords:
granulocyte colony-stimulating factor;
dendritic cells;
T-lymphocytes, helper-inducer;
immune response
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of granulocyte colony-stimulating factor (G-CSF) in regulating dendritic cells (DC) subsets and its effect on immune response. Methods BALB/c mice received 10?g of G-CSF once a day by subcutaneous injection for 6 days. Then, DC were separated from the peripheral blood. The ratio of DC1(CD11c +CD8a -) and DC2(CD11c +CD8a +) and their absolute numbers were determined with flow cytometry. One week after C57BL/6 PBMC stimulation, mixed lymphocyte culture was used to determine the ability of DC to proliferate priming T cells and the intensity of mixed lymphocyte reaction (MLR). Results After stimulation with different doses of G-CSF for 6 days, the absolute number of DC2 was increased from 9.6?10 6/L to 45.4?9.9?10 6/L (P
