MYOCARDIAL FIBROSIS INDUCED BY HIGH-CHOLESTEROL DIET AND ITS MECHANISM
- VernacularTitle:高胆固醇饮食诱导的心肌纤维化及其发生机制
- Author:
Xiaohua XIE
;
Ning LIU
;
Xiuhua LIU
- Publication Type:Journal Article
- Keywords:
endomyocardial fibrosis;
cholesterol;
aldosterone;
mitogen activated protein kinase(MAPK)
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study induction of myocardial fibrosis by high-cholesterol diet and its mechanism in rat. Methods Nineteen Wister rats aged 8 weeks were randomly divided into control group, high-cholesterol diet group and spironolactone treatment group. Collagen in left ventricular tissues was determined by oxyproline assay, contents of angiotensin Ⅱ(Ang Ⅱ) and aldosterone (Ald) and in left ventricular tissue their plasm concentration were determined by radioimmunoassay. Serum nitrite level was measured by Griess assay. Mitogen activated protein kinase (MAPK) was assessed by radioisotope labeled assay. Results The contents of collagen, cardiac Ald and plasma Ang Ⅱ in the myocardium of the left ventricle were increased 1.2, 1.1 and 3.0 fold, respectively, while serum level of nitrite (NO - 2) was significantly decreased in high-cholesterol diet group compared with control group. The contents of myocardial collagen and Ald, and plasma Ang Ⅱ and the activities of MAPK were decreased in high-cholesterol diet group treated with spironolactone for 8 weeks (P
