Detection of the Microdeletion on the Y Chromosome of Patients with Idiopathic Oligospermia or Azoospermia.
- Author:
Mi Hyang KIM
1
Author Information
1. Department of Laboratory Medicine, College of Medicine, Kosin University, Busan, Korea. mihakim@ns.kosinmed.or.kr
- Publication Type:Original Article
- Keywords:
Y chromosome;
Microdeletion;
Azoospermia;
Oligospermia;
Azoospermia factor
- MeSH:
Arm;
Azoospermia*;
Chromosomes, Human, Y;
DNA;
Humans;
Incidence;
Male;
Oligospermia*;
Polymerase Chain Reaction;
RNA;
Spermatogenesis;
Y Chromosome*
- From:The Korean Journal of Laboratory Medicine
2002;22(6):452-456
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: It has been proposed that the long arm of the human Y chromosome contains AZF (the azoospermia factor), the gene or genes that control spermatogenesis. In this study, I detected microdeletions on the long arm of the Y chromosome and analysed the relationship between the microdeletion detected and the failure of spermatogenesis in the patients investigated. METHODS: In this study, I analyzed 35 infertile patients, including 21 azoospermia and 14 oligospermia. Genomic DNAs were isolated from peripheral blood samples. Each sample was examined for the presence or absence of the total 9 Y-DNA landmarks on the Y chromosome including those deleted in the azoospermia and Y-chromosome RNA recognition motif (RBM1), using the polymerase chain reaction amplification. RESULTS: I detected microdeletions on the long arm of the Y chromosome in 4 patients with azoospermia. All 4 samples with microdeletions of the Y chromosome were identified with microdeletions of multiple loci. The microleletion incidence was 2.9% for sY143 and 11.4% for other loci (sY152, sY153 and sY255). But, the microdeletion of RBM1 was not identified. CONCLUSIONS: Even though the microdeletion analysis of the Y chromosome was not fully performed, this report suggests the presence of microdeletions within the Y chromosome in patients with azoospermia, supporting the relationship between the chromosomal region involved and the process of sper-matogenesis.
