COMPARISON OF DENDRITIC CELL DIFFERENTIATION AND MATURATION IN VITRO IN PERIPHERAL BLOOD MONONUCLEAR CELL CULTURES OF NORMAL SUBJECTS AND PATIENTS WITH GASTROINTESTINAL CARCINOMA
- VernacularTitle:胃肠道腺癌患者与正常人PBMC诱生DC差异性研究
- Author:
Zheng PENG
;
Rong LI
;
Li LI
- Publication Type:Journal Article
- Keywords:
adenocarcinoma;
monocytes;
dendritic cells
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether gastrointestinal carcinoma could influence differentiation and maturation of dentritic cell (DC) in cultured peripheral blood mononuclear cells (PBMC). Methods PBMC of peripheral blood obtained either from healthy individuals or gastrointestinal carcinoma patients were incubated to induce into DC with the aid of addition of GM-CSF, IL-4 and TNF-?. The percentage of DC and expression rate of markers CD83, CD1a were determined by flow cytometric analysis. Results The quantity of PBMC isolated from gastrointestinal carcinoma patients was less than that from healthy individuals with same volume of blood. DC could be induced from PBMC of gastrointestinal carcinoma patients, and they could express B7 costimulatory molecules as those of healthy individuals. But the expression rates of CD83, CD1a were significantly different in two groups. Conclusion The quantity of PBMC and their ability to differentiate into DC seemed to be decreased in gastrointestinal carcinoma patients.
