DETECTION OF GENE MUTATIONS IN HBV PRECORE AND BASIC CORE PROMOTER REGION USING MICROARRAY TECHNIQUE
- VernacularTitle:应用基因芯片技术检测乙型肝炎病毒前-C区/BCP区基因突变的研究
- Author:
Xiumei ZHOU
;
Xun QU
;
Fuli LIU
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
gene, pre-core/BCP region;
mutation;
gene chip
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of micro-array technique in the detection of HBV pre-core and basic core promoter region variants. Methods Four spot mutations of A1896(nt1896G→A) and A1899( nt1899G→A) in precore region and T1762A1764(nt1762A→T,nt1764G→A) in basic core promoter region in 46 patients of acute and chronic liver diseases were detected by gene chips to investigate the clinical value of micro-array technique. Results Micro-array technique had a high specificity in the detection of specific mutation, and the positive rate was 87.0%. A1896 mutation was found in 18 cases (45.0%), A1899 mutation in 10 cases (25.0%), T1762 and A1764 double variants in 30 cases (75.0%), and poly-sites variants in 14 cases (35.0%). Significant difference in liver functions was found between each mutation group and non-mutation group (P
