Effect of OX-LDL on proliferation of vascular smooth muscles and activities of human a2( I ) procollagen gene promotor
- VernacularTitle:OX-LDL对血管平滑肌细胞增殖及人Ⅰ型胶原?2(Ⅰ)基因启动子活性的影响
- Author:
Qian CHEN
;
Zonggui WU
;
Chunfang GAO
;
Hao WANG
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
OX-LDL;
vascular smooth muscle cell;
collagen;
promotor
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of OX-LDL on the formation and progression of atherosclerosis. Methods: (1) The thoracic aorta smooth muscle cells(VSMC) of male Sprague-Dawley rat were primarily cultured in vitro. Different concentrations of OX-LDL(50,100,150,200 ?g/ml) were co-incubated with the cells respectively. MTT method was used to detect the cell proliferation after 24, 48 and 72 h. (2) pCOLH22. 4 and pCOLH2l. 6, recombinants of human a2( I ) procol-lagen gene 5' flank sequence ( - 2. 4 kb and -1. 6 kb in size) and CAT reporter gene, were used to transiently transfect smooth muscle cells with FuGENE Transfectant Reagent. The effect of OX-LDL (150 ?g/ml) on the plasmid were determined by CAT-EL1SA. Results: (1)OX-LDL accelerated the proliferation of VSMC in dose-dependent manner. (2)OX-LDL enhanced the promoter activities of human a2( I ) procollagen gene significantly. Conclusion: OX-LDL can accelerate the proliferation of VSMC and the formation of type I collagen,which facilitates the formation and progression of atherosclerosis.
