Procaryotic expression of human FceR I a subunit extracellular domain and its binding mechanism with IgE
- VernacularTitle:人Fc?RI?亚基细胞外区的原核表达及其和IgE结合的机制
- Author:
Danrong YANG
;
Qingyu XIU
;
Huanxing HAN
- Publication Type:Journal Article
- Keywords:
Fc receptor;
gene expression;
IgE
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Human FceR I a subunit extracellular domain was cloned and expressed with 2 different expressing systems and Dot blot was used to detect its biological activity to bind with IgE,providing reference for binding mechanism of human FceR I a subunit extracellular domain with IgE. It was shown that FceR I a subunit extracellular domain from pBAD/g I A expressing system could bind with IgE, but the one from PQE30 expressing system could not bind with IgE. It is suggested that FceR I a subunit extracellular domain alone is sufficient to bind with IgE without P and Y subunit. The proper space configuration and disulfide bond of FceR I a subunit is necessary for binding with IgE, but its glycosylation is unnecessary.
