Effects of homoharringtonine (HHT) on expression of caspase-3 protein and mRNA in nasopharyrgeal carcinoma cells CNE-2Z
- VernacularTitle:HHT对CNE-2Z细胞caspase-3的蛋白和mRNA表达的影响
- Author:
Keyuan ZHOU
;
Xudong TANG
;
Hang DING
;
Suzhe CHEN
- Publication Type:Journal Article
- Keywords:
homoharringtonine(HHT);
nasopharyngeal carcinoma;
caspase-3;
protein;
mRNA
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of homoharringtonine (HHT) on the expression of caspase-3 pro-tein and mRNA in nasopharyngeal carcinoma cells CNE-2Z. METHODS After CNE-2Z cells were treated with HHT [0(control),0.125,0.25,0.5,1 mg?L -1] for 8 h, the expression of pro-caspase-3 protein was analyzed by Western Blot and the expression of caspase-3 mRNA was detected by semi-quantitative RT-PCR. RESULTS As HHT-concentration increased, the expression of pro-caspase-3 protein decreased significantly (P
