Effect of rubescensine B on apoptosis and Bcl-2、p53、Fas/APO-1、C-myc expression in GBC-SD cells
- VernacularTitle:冬凌草乙素诱导GBC-SD细胞的凋亡及对Bcl-2、p53、Fas/APO-1、C-myc表达的影响
- Author:
Hongwei XUE
;
Xiangli PAN
- Publication Type:Journal Article
- Keywords:
rubescensine B;
GBC-SD;
apoptosis;
Bcl-2;
p53;
Fas/APO-1;
C-myc
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the inhibition and apoptosis mechanism of GBC-SD cells induced by rubescensine B. METHODS Using MTT, convert microscopy, electron microscopy, flow cytometry, an immunohistochemical assay, and spectrofluorometry demonstrate the presence and pathogenesis of apoptosis after treated by rubescensine B. RESULTS After exposure to Rubecensine B GBC-SD cells were induced to apoptosis in dose-dependent manner, and the level of Bcl-2,p53,C-myc,Fas/APO-1 were decreased within 24 hours, reversely the activity of Caspase-3 was enhanced with the appearance of apoptosis. CONCLUSION Rubecensine B can induce GBC-SD cells apoptosis related to Bcl-2,p53,Fas/APO-1 and C-myc.
